6L6Q
Structural basis of NR4A2 homodimers binding to selective Nur-responsive elements
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 43 |
Unit cell lengths | 35.696, 35.696, 259.273 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.409 - 2.601 |
R-factor | 0.2553 |
Rwork | 0.249 |
R-free | 0.28920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cit |
RMSD bond length | 0.006 |
RMSD bond angle | 0.866 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.410 | 2.693 |
High resolution limit [Å] | 2.601 | 2.601 |
Rmerge | 0.075 | 0.555 |
Rmeas | 0.077 | |
Number of reflections | 9915 | 990 |
<I/σ(I)> | 16.81 | 4.42 |
Completeness [%] | 100.0 | |
Redundancy | 13.8 | |
CC(1/2) | 1.000 | 0.971 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 150 mM NaCl, 50 mM MES (pH 5.93), 10 mM MgCl2, 5 mM CaCl2, and 10-12% PEG4K (w/v) |