6L6M
HSP18.5 from E. histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.991872 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.237, 173.988, 159.058 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.529 - 3.280 |
| R-factor | 0.189283205598 |
| Rwork | 0.187 |
| R-free | 0.22751 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w1z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.049 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.530 | 3.430 |
| High resolution limit [Å] | 3.280 | 3.280 |
| Number of reflections | 15560 | 2004 |
| <I/σ(I)> | 5.99 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 11.1 | |
| CC(1/2) | 0.990 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | 0.1 M Bis-Tris 160 mM ammonium acetate 45% 2-Methyl-2,4-pentanediol |
