6L6D

X-ray structure of human galectin-10 in complex with D-N-acetylgalactosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-08-28
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	48.900, 48.900, 261.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.628 - 1.930
Rwork	0.202
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qkq
RMSD bond length	0.006
RMSD bond angle	1.363
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.630	1.980
High resolution limit [Å]	1.930	1.930
Number of reflections	14992	1048
<I/σ(I)>	16.8
Completeness [%]	99.8
Redundancy	19.3
CC(1/2)	0.998	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6 M ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 10 % (v/v) 1,4-dioxane