6L64

X-ray structure of human galectin-10 in complex with D-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-12-06
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	48.650, 48.650, 259.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.517 - 2.080
Rwork	0.199
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qkq
RMSD bond length	0.006
RMSD bond angle	1.539
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.520	2.130
High resolution limit [Å]	2.080	2.080
Number of reflections	11869	832
<I/σ(I)>	15.1	4.1
Completeness [%]	99.8
Redundancy	19.4
CC(1/2)	0.998	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6 M ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 10 % (v/v) 1,4-dioxane