6L5S
crystal structure of GgCGT in complex with UDP-Glu
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.376, 70.620, 108.402 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.484 - 1.914 |
R-factor | 0.1604 |
Rwork | 0.158 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2acw |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.930 |
High resolution limit [Å] | 1.900 | 5.150 | 1.900 |
Rmerge | 0.166 | 0.066 | 0.773 |
Rmeas | 0.176 | 0.070 | 0.820 |
Rpim | 0.056 | 0.022 | 0.269 |
Total number of observations | 293646 | ||
Number of reflections | 31904 | 1699 | 1545 |
<I/σ(I)> | 3.9 | ||
Completeness [%] | 99.5 | 96.6 | 100 |
Redundancy | 9.2 | 8.9 | 8.8 |
CC(1/2) | 0.998 | 0.779 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium acetate, 0.1 M Tris, pH 8.5, 25% w/v Polyethylene glycol 3350 |