6L5R

crystal structure of GgCGT in complex with UDP-Glu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	53.750, 67.336, 105.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.668 - 2.890
R-factor	0.2084
Rwork	0.206
R-free	0.26110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2acw
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.950
High resolution limit [Å]	2.890	7.850	2.900
Rmerge	0.197	0.068	0.848
Rmeas	0.210	0.073	0.940
Rpim	0.072	0.026	0.395
Number of reflections	9044	513	419
<I/σ(I)>	3.5
Completeness [%]	99.7	100	96.5
Redundancy	8.1	7.6	5.2
CC(1/2)		0.996	0.427

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium acetate, 0.1 M Tris, pH 8.5, 25% w/v Polyethylene glycol 3350