6L5O
Crystal structure of human DEAD-box RNA helicase DDX21 at post-hydrolysis state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97892 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.460, 43.830, 72.440 |
Unit cell angles | 90.00, 115.64, 90.00 |
Refinement procedure
Resolution | 36.906 - 1.800 |
R-factor | 0.1773 |
Rwork | 0.175 |
R-free | 0.20960 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.865 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 65.307 | 36.906 | 1.900 |
High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
Rmerge | 0.046 | 0.673 | |
Rmeas | 0.145 | 0.052 | 0.765 |
Rpim | 0.065 | 0.023 | 0.354 |
Total number of observations | 139159 | 4453 | 19644 |
Number of reflections | 33164 | 1037 | 4904 |
<I/σ(I)> | 7.9 | 18.1 | 2.3 |
Completeness [%] | 94.6 | 89 | 96.1 |
Redundancy | 4.2 | 4.3 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% pEG3350, 0.2m Magnesium acetate |