6L5A

The structure of the UdgX mutant H109E at a pre-excision state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-11
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	47.698, 48.316, 86.222
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.555 - 1.800
R-factor	0.153380596451
Rwork	0.151
R-free	0.19267
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6io9
RMSD bond length	0.012
RMSD bond angle	1.668
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.560	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.064	0.128
Number of reflections	18687	2586
<I/σ(I)>	15.9	8.4
Completeness [%]	98.1	95.1
Redundancy	4.8	4.2
CC(1/2)	0.998	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	19% PEG3350, 0.1 M sodium citrate, 0.2 M NaCl