6L3G
Structural Basis for DNA Unwinding at Forked dsDNA by two coordinating Pif1 helicases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-30 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 131.265, 131.265, 117.183 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.566 - 3.300 |
R-factor | 0.2472 |
Rwork | 0.244 |
R-free | 0.30310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fhd |
Data reduction software | XFIT |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.700 | 3.560 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.200 | |
Number of reflections | 17102 | 3547 |
<I/σ(I)> | 7.1 | |
Completeness [%] | 97.9 | |
Redundancy | 6.2 | |
CC(1/2) | 0.982 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 80 mM Sodium chloride, 40 mM Sodium cacodylate pH 6.0, 35% MPD, 12 mM Spermine |