6L39
Cytochrome P450 107G1 (RapN)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.978, 99.798, 62.684 |
| Unit cell angles | 90.00, 89.82, 90.00 |
Refinement procedure
| Resolution | 32.770 - 2.970 |
| R-factor | 0.2049 |
| Rwork | 0.198 |
| R-free | 0.31320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z5p |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 39493 | 39493 |
| <I/σ(I)> | 8.6 | 8.6 |
| Completeness [%] | 96.6 | |
| Redundancy | 2.8 | |
| CC(1/2) | 0.949 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 287.15 | 0.1 M imidazole pH 8.0 1 M sodium citrate |
