6L39

Cytochrome P450 107G1 (RapN)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97940
Spacegroup name	P 1 21 1
Unit cell lengths	59.978, 99.798, 62.684
Unit cell angles	90.00, 89.82, 90.00

Refinement procedure

Resolution	32.770 - 2.970
R-factor	0.2049
Rwork	0.198
R-free	0.31320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z5p
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Number of reflections	39493	39493
<I/σ(I)>	8.6	8.6
Completeness [%]	96.6
Redundancy	2.8
CC(1/2)	0.949	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	287.15	0.1 M imidazole pH 8.0 1 M sodium citrate