6L33

Crystal structure of the regulatory domain of MexT, a transcriptional activator in Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97960
Spacegroup name	P 21 21 21
Unit cell lengths	65.652, 108.703, 109.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.840 - 2.000
R-factor	0.2079
Rwork	0.206
R-free	0.25300
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.665
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.040	0.490
Rmeas	0.059	0.400
Rpim	0.023	0.190
Number of reflections	53533	2444
<I/σ(I)>	23.76	2.91
Completeness [%]	97.1	93.6
Redundancy	5.6	3.7
CC(1/2)		0.633

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.5 M Ammonium sulfate, 0.1 M lithium sulfate, 1.0 M sodium citrate tribasic dehydrate (pH 5.6), 2 mM EDTA