6L2W
Crystal structure of a novel fold protein Gp72 from the freshwater cyanophage Mic1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.702, 45.534, 67.368 |
Unit cell angles | 90.00, 94.29, 90.00 |
Refinement procedure
Resolution | 42.770 - 2.290 |
R-factor | 0.2012 |
Rwork | 0.199 |
R-free | 0.23850 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.239 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.290 | 2.300 |
Rmerge | 0.083 | 0.301 |
Number of reflections | 11607 | 1125 |
<I/σ(I)> | 25.23 | 4.9 |
Completeness [%] | 98.8 | |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 13% polyethylene glycol 6000, 0.1 M ADA pH 7.0, 0.01 M spermine tetrahydrochloride and 0.1 M guanidine hydrochloride |