6L2U

Soluble methane monooxygenase reductase FAD-binding domain from Methylosinus sporium.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-15
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	34.590, 59.872, 94.499
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.500
R-factor	0.1617
Rwork	0.160
R-free	0.19720
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.410
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	32235	1563
<I/σ(I)>	61.9
Completeness [%]	99.8
Redundancy	14
CC(1/2)	0.996	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	295	10% PEG 3000, 0.1M Na-phosphate dibasic/citric acid pH 4.2, 0.2M NaCl