6L2M

The structure of the tRNA-specific deaminase mutant from M. capricolum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	55.589, 55.589, 109.227
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.141 - 2.303
R-factor	0.201104412273
Rwork	0.198
R-free	0.25141
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l2l
RMSD bond length	0.007
RMSD bond angle	0.813
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.105	0.510
Number of reflections	9158	889
<I/σ(I)>	38.5	4.8
Completeness [%]	100.0	100
Redundancy	18.7	18.1
CC(1/2)	0.990	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	1.4M ammonium sulfate, 0.1M Sodium citrate pH 6.0