6L2L
The structure of the tRNA-specific deaminase from M. capricolum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-05-07 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 49.138, 49.138, 147.931 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.065 - 2.400 |
R-factor | 0.190815182497 |
Rwork | 0.189 |
R-free | 0.22051 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a8n |
RMSD bond length | 0.005 |
RMSD bond angle | 0.682 |
Data reduction software | CrysalisPro |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.070 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.100 | 0.460 |
Number of reflections | 7697 | 1081 |
<I/σ(I)> | 15.6 | 4.5 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.6 | 7.1 |
CC(1/2) | 0.998 | 0.884 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 277 | 0.49M sodium phosphate monobasic monohydrate, 0.91M potassium phosphate dibasic, pH 6.9 |