6L2A
A mutant form of M. tb toxin MazEF-mt1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION SUPERNOVA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-09-25 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 42.313, 42.313, 87.612 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.565 - 1.900 |
| R-factor | 0.19884316004 |
| Rwork | 0.197 |
| R-free | 0.23280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kys |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.601 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.900 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.089 | 0.973 |
| Number of reflections | 7614 | 1086 |
| <I/σ(I)> | 17 | 1.8 |
| Completeness [%] | 99.9 | |
| Redundancy | 8.3 | |
| CC(1/2) | 0.998 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20% poly (ethylene glycol) 3350, 0.1 M NaOAc (pH 5.0), and 0.1 M NaCl |
