6L2A
A mutant form of M. tb toxin MazEF-mt1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION SUPERNOVA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-09-25 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 42.313, 42.313, 87.612 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.565 - 1.900 |
R-factor | 0.19884316004 |
Rwork | 0.197 |
R-free | 0.23280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6kys |
RMSD bond length | 0.003 |
RMSD bond angle | 0.601 |
Data reduction software | CrysalisPro |
Data scaling software | CrysalisPro |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.900 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.089 | 0.973 |
Number of reflections | 7614 | 1086 |
<I/σ(I)> | 17 | 1.8 |
Completeness [%] | 99.9 | |
Redundancy | 8.3 | |
CC(1/2) | 0.998 | 0.669 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20% poly (ethylene glycol) 3350, 0.1 M NaOAc (pH 5.0), and 0.1 M NaCl |