6L1Y
structure of gp120/CD4 with a non-canonical surface
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.444, 66.468, 73.850 |
Unit cell angles | 90.00, 108.40, 90.00 |
Refinement procedure
Resolution | 33.815 - 2.469 |
R-factor | 0.2061 |
Rwork | 0.203 |
R-free | 0.25680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tgt |
RMSD bond length | 0.009 |
RMSD bond angle | 1.342 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL2Map |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.469 | 2.470 |
Rmerge | 0.050 | 0.470 |
Number of reflections | 21690 | 1803 |
<I/σ(I)> | 21.6 | 2.15 |
Completeness [%] | 95.6 | 96.8 |
Redundancy | 3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.4 | 293 | 0.1 M HEPES sodium, pH 7.4, 11% PEG 4000 and 10% 2-propanol |