6L1Q
Crystal structure of AfCbbQ2, a MoxR AAA+-ATPase and CbbQO-type Rubisco activase from Acidithiobacillus ferrooxidans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.05 |
Spacegroup name | P 6 |
Unit cell lengths | 167.753, 167.753, 48.291 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.056 - 2.200 |
R-factor | 0.197 |
Rwork | 0.194 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5c3c |
RMSD bond length | 0.005 |
RMSD bond angle | 0.945 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.090 | 0.360 |
Rpim | 0.040 | 0.180 |
Number of reflections | 56135 | 8038 |
<I/σ(I)> | 16.1 | 5.1 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 10.5 | 9.7 |
CC(1/2) | 0.990 | 0.950 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.2 | 293 | 25% (v/v) 1,2-propanediol, phosphate-citrate pH4.2, 5% (v/v) PEG3000, 10% (v/v) glycerol |