6L1N
Substrate bound BacF structure from Bacillus subtillis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9840 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.290, 56.910, 78.500 |
| Unit cell angles | 90.00, 97.86, 90.00 |
Refinement procedure
| Resolution | 77.760 - 2.000 |
| R-factor | 0.1844 |
| Rwork | 0.182 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o1b |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.585 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.760 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 42411 | 4243 |
| <I/σ(I)> | 15.46 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.991 | 0.737 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 0.1M sodium acetate trihydrate, 0.1M cacodylate trihydrate (pH= 6.5), 30% w/v polyethylene glycol |
