6L1M
Structure of human StAR-related lipid transfer protein 4 mutant - LWNI107-110GG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.914, 43.357, 89.798 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.020 - 1.700 |
R-factor | 0.1686 |
Rwork | 0.168 |
R-free | 0.19010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6l1d |
RMSD bond length | 0.005 |
RMSD bond angle | 0.794 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.088 | 0.432 |
Number of reflections | 19187 | 943 |
<I/σ(I)> | 33.8 | 5.5 |
Completeness [%] | 99.3 | |
Redundancy | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 287 | 0.1M MES pH 6.0, 25% PEG 3350, 200 mM NaCl |