6L1L
Apo-BacF structure from Bacillus subtillis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.220, 59.440, 84.230 |
| Unit cell angles | 90.00, 101.78, 90.00 |
Refinement procedure
| Resolution | 82.460 - 1.900 |
| R-factor | 0.2292 |
| Rwork | 0.227 |
| R-free | 0.27790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o1b |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.504 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.460 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 55815 | 55815 |
| <I/σ(I)> | 4.13 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3 | |
| CC(1/2) | 0.985 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 0.1M sodium acetate trihydrate, 0.1M sodium cacodylate trihydrate at pH 6.5, 30% w/v polyethylene glycol 8000 |
