6L1G

Crystal structure of light-dependent protochlorophyllide oxidoreductase from Synechocystis sp. PCC 6803

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	156.010, 57.122, 72.856
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.086 - 2.200
R-factor	0.1801
Rwork	0.179
R-free	0.20740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rd5
RMSD bond length	0.004
RMSD bond angle	0.727
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.107
Rpim	0.052	0.428
Number of reflections	33980	3338
<I/σ(I)>	15.2
Completeness [%]	99.9	99.9
Redundancy	4.8	4.9
CC(1/2)	0.990	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	289	0.1 M HEPES, pH 7.5, 1.5 M lithium sulfate