6L1F

Crystal structure of PHF20L1 Tudor1 in complex with K142me1 DNMT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	42.702, 52.932, 31.394
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.235 - 1.900
R-factor	0.196
Rwork	0.192
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sd4
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.240	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.119	0.541
Number of reflections	5921	553
<I/σ(I)>	16.9	2.1
Completeness [%]	99.0
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30% PEG MME 2000, 0.1M sodium cacodylate, pH 6.0