6L1E

Crystal structure of middle domain of hSSRP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-19
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	39.002, 66.210, 84.501
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.300 - 2.094
R-factor	0.1878
Rwork	0.186
R-free	0.22450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gcj
RMSD bond length	0.007
RMSD bond angle	0.865
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.300	2.180
High resolution limit [Å]	2.094	2.094
Rmerge	0.087	0.376
Number of reflections	13367	1270
<I/σ(I)>	26.94
Completeness [%]	99.1
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	10mM MES pH4.0, 12.5% 2-propanol, 20%(w/v) PEG 2000