6L1D

Structure of human StAR-related lipid transfer protein 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-01
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97950
Spacegroup name	P 41
Unit cell lengths	93.770, 93.770, 73.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.750 - 1.950
R-factor	0.1892
Rwork	0.188
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jss
RMSD bond length	0.006
RMSD bond angle	0.759
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.073	0.440
Number of reflections	45852	2304
<I/σ(I)>	32.5	4.2
Completeness [%]	98.7
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	287	0.1 M Bicine pH 9.0, 30% PEG 1000