6L1C
Crystal Structure Of of PHF20L1 Tudor1 Y24L mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 |
Unit cell lengths | 52.570, 52.570, 38.646 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.170 - 1.580 |
R-factor | 0.1792 |
Rwork | 0.178 |
R-free | 0.19090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sd4 |
RMSD bond length | 0.003 |
RMSD bond angle | 1.161 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.173 | 1.623 |
High resolution limit [Å] | 1.580 | 1.582 |
Rmerge | 0.097 | 0.341 |
Number of reflections | 13228 | 956 |
<I/σ(I)> | 37.4 | 6.3 |
Completeness [%] | 99.9 | |
Redundancy | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.6M lithium sulfate, 0.1M Tris, PH 8.0 |