6L19

The crystal structure of competence or damage-inducible protein from Enterobacter asburiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	128.915, 128.915, 56.277
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.230 - 2.130
R-factor	0.1875
Rwork	0.185
R-free	0.22100
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.883
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.170
High resolution limit [Å]	2.130	2.130
Number of reflections	27236	790
<I/σ(I)>	21.6	2.6
Completeness [%]	100.0	100
Redundancy	24.8	19.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	287	2.5 M Ammonium phosphate monobasic, 0.1 M Tric-HCl