6L11
Crystal structure of Ser/Thr kinase Pim1 in complex with 10-DEBC derivatives
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Collection date | 2013-08-26 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 0.97853 |
Spacegroup name | P 65 |
Unit cell lengths | 97.915, 97.915, 80.718 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 84.800 - 2.050 |
R-factor | 0.2143 |
Rwork | 0.213 |
R-free | 0.23980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ywv |
RMSD bond length | 0.018 |
RMSD bond angle | 1.906 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.800 | 2.103 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.074 | 0.262 |
Number of reflections | 27687 | 26248 |
<I/σ(I)> | 5.9 | |
Completeness [%] | 99.7 | 99.46 |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.4M potassium sodium tartrate tetrahydrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |