6L10
PHF20L1 Tudor1 - MES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.175, 52.773, 101.375 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.103 - 1.600 |
| R-factor | 0.1955 |
| Rwork | 0.194 |
| R-free | 0.22090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sd4 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (3.3.22) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.103 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.073 | 0.420 |
| Number of reflections | 38337 | 3702 |
| <I/σ(I)> | 24.8 | 3.5 |
| Completeness [%] | 99.8 | |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 8000, 0.2M (NH4)2SO4, 0.1mM MES, PH 6.5 |
