6L0W
Structure of RLD2 BRX domain bound to LZY3 CCL motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-06 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9658 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.755, 55.658, 93.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.763 - 1.591 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22440 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.972 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.13_2998: ???) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.620 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.083 | 0.457 |
| Number of reflections | 26622 | 2261 |
| <I/σ(I)> | 17.4 | 2.2 |
| Completeness [%] | 98.1 | 88 |
| Redundancy | 6.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | PEG 4000, 0.05 M sodium citrate buffer (pH 6.5), 0.1 M sodium acetate |
