6L0K
Crystal structure of dihydroorotase in complex with malate at pH9 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-17 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.824, 88.856, 103.751 |
| Unit cell angles | 90.00, 95.38, 90.00 |
Refinement procedure
| Resolution | 46.156 - 3.300 |
| R-factor | 0.2168 |
| Rwork | 0.214 |
| R-free | 0.26560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6l0a |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.156 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 22773 | 1822 |
| <I/σ(I)> | 17.7 | |
| Completeness [%] | 96.4 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.931 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 100mM MMT/sodium hydroxide pH 9, 26% PEG 1500 |
