6L0J
Crystal structure of Dihydroorotase in complex with malate at pH7.5 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.553, 88.337, 103.195 |
| Unit cell angles | 90.00, 95.60, 90.00 |
Refinement procedure
| Resolution | 28.305 - 1.933 |
| R-factor | 0.1848 |
| Rwork | 0.183 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6l0a |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.305 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 100526 | 2710 |
| <I/σ(I)> | 18.17 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.875 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 100 mM imidazole malate pH 7.5, 19% PEG 4000 |
