6L0H
Crystal structure of dihydroorotase in complex with malate at pH7 from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-04-19 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.569, 88.521, 103.293 |
Unit cell angles | 90.00, 95.42, 90.00 |
Refinement procedure
Resolution | 31.965 - 2.054 |
R-factor | 0.1848 |
Rwork | 0.183 |
R-free | 0.22220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6l0a |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.965 | 2.130 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 87644 | 3931 |
<I/σ(I)> | 17.5 | |
Completeness [%] | 91.5 | |
Redundancy | 4.9 | |
CC(1/2) | 0.908 | 0.583 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 100mM imidazole malate pH 7, 20% PEG 4000 |