6L03
structure of PTP-MEG2 and MUNC18-1-pY145 peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2019-03-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9788 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 71.048, 84.074, 48.753 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.266 - 2.084 |
R-factor | 0.2113 |
Rwork | 0.210 |
R-free | 0.23040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4icz |
RMSD bond length | 0.008 |
RMSD bond angle | 0.968 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.300 | 2.200 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.191 | 0.927 |
Number of reflections | 18019 | 1773 |
<I/σ(I)> | 6.5 | |
Completeness [%] | 99.8 | |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 4000, 0.2M KSCN, 10% ethelene glycol, 0.1M bis-tris propane |