6KZW

Crystal structure of YggS family pyridoxal phosphate-dependent enzyme PipY from Fusobacterium nucleatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	37.929, 146.375, 74.128
Unit cell angles	90.00, 93.36, 90.00

Refinement procedure

Resolution	37.000 - 2.080
R-factor	0.1745
Rwork	0.173
R-free	0.21740
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.922
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.080	2.080
Number of reflections	47064	1000
<I/σ(I)>	27.13
Completeness [%]	96.1
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M Ammonium sulfate 20% w/v polyethylene glycol 4,000 0.1 M Sodium acetate trihydrate(pH 4.6)