6KZW
Crystal structure of YggS family pyridoxal phosphate-dependent enzyme PipY from Fusobacterium nucleatum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-07-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.929, 146.375, 74.128 |
Unit cell angles | 90.00, 93.36, 90.00 |
Refinement procedure
Resolution | 37.000 - 2.080 |
R-factor | 0.1745 |
Rwork | 0.173 |
R-free | 0.21740 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.922 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.080 | 2.080 |
Number of reflections | 47064 | 1000 |
<I/σ(I)> | 27.13 | |
Completeness [%] | 96.1 | |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M Ammonium sulfate 20% w/v polyethylene glycol 4,000 0.1 M Sodium acetate trihydrate(pH 4.6) |