6KZV
Crystal structure of E.coli DNA gyrase B in complex with 2-oxo-1,2-dihydroquinoline derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-01-29 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 |
Unit cell lengths | 53.444, 53.444, 67.884 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.900 - 2.400 |
R-factor | 0.19859 |
Rwork | 0.196 |
R-free | 0.26032 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aj6 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.900 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.081 | 0.348 |
Number of reflections | 7467 | 733 |
<I/σ(I)> | 12.6 | |
Completeness [%] | 99.3 | |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | MES, Magnesium chloride, PEG4000 |