6KZT
Crystal structure of cytochrome P450mel 107F1 with biaryl coupling reactivity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-20 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 |
Unit cell lengths | 117.508, 117.508, 75.164 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.170 - 3.500 |
R-factor | 0.1968 |
Rwork | 0.188 |
R-free | 0.28140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d09 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.679 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.560 |
High resolution limit [Å] | 3.500 | 9.480 | 3.500 |
Rmerge | 0.236 | 0.056 | 0.554 |
Rmeas | 0.242 | 0.057 | 0.567 |
Rpim | 0.054 | 0.013 | 0.124 |
Total number of observations | 151136 | ||
Number of reflections | 7557 | 400 | 383 |
<I/σ(I)> | 3.5 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 20 | 18.7 | 20.9 |
CC(1/2) | 0.999 | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 18% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 4000 |