6KZR

Crystal structure of mouse DCAR2 CRD domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-25
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	65.979, 72.435, 101.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.971 - 2.304
Rwork	0.189
R-free	0.22070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lfj
RMSD bond length	0.007
RMSD bond angle	1.407
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.900	2.440
High resolution limit [Å]	1.840	2.300
Rmerge	0.120	0.687
Number of reflections	22002	3468
<I/σ(I)>	15.1	2.99
Completeness [%]	99.7
Redundancy	7.2
CC(1/2)		0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	PEG