6KZH

14-3-3 protein in Complex with CIC S173 phosphorylated peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	70.169, 80.810, 107.064
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.628 - 2.645
R-factor	0.2212
Rwork	0.215
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5KZG
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.628	2.740
High resolution limit [Å]	2.645	2.645
Rmerge	0.147	1.270
Number of reflections	18361	1800
<I/σ(I)>	11.1
Completeness [%]	99.8
Redundancy	12.8
CC(1/2)	1.000	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Sodium chloride, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350