6KZG

14-3-3 protein in Complex with CIC S301 phosphorylated peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	69.657, 79.487, 106.261
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.170 - 2.000
Rwork	0.191
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iqp
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.170	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.113
Number of reflections	40758	3950
<I/σ(I)>	16.9
Completeness [%]	99.8
Redundancy	12.8
CC(1/2)	1.000	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	8% v/v TacsimateTM pH 6.0, 20% w/v Polyethylene glycol 3350