6KZ6

Crystal structure of ASFV dUTPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	66.256, 67.278, 116.912
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.128 - 2.187
R-factor	0.1976
Rwork	0.196
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.220
High resolution limit [Å]	2.180	2.180
Number of reflections	27729	1349
<I/σ(I)>	10.5
Completeness [%]	100.0
Redundancy	12.9
CC(1/2)	0.990	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.08 M HEPES pH 7.5, 0.02 M sodium citrate tribasic dihydrate, 17% w/v Polyethylene glycol 3350