6KZ1

Complex structure of Whirlin and Myosin XVa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	P 31 2 1
Unit cell lengths	51.974, 51.974, 70.445
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.011 - 1.694
R-factor	0.1798
Rwork	0.179
R-free	0.20080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ufx
RMSD bond length	0.012
RMSD bond angle	1.424
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.011	45.011	1.800
High resolution limit [Å]	1.690	5.050	1.690
Rmerge	0.055	0.035	0.685
Rmeas	0.057	0.037	0.722
Total number of observations	242635
Number of reflections	23647	892	3810
<I/σ(I)>	22.54	57.71	3.12
Completeness [%]	99.8	100	98.7
Redundancy	10.261	10.483	9.813
CC(1/2)	0.999	0.999	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Potassium phosphate dibasic, HEPES pH7.5, Sodium phosphate monobasi