6KZ1
Complex structure of Whirlin and Myosin XVa
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 31 2 1 |
| 格子定数 [Å] | 51.974, 51.974, 70.445 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 分解能 | 45.011 - 1.694 |
| R因子 | 0.1798 |
| Rwork | 0.179 |
| R-free | 0.20080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ufx |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.424 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 45.011 | 45.011 | 1.800 |
| 分解能 [Å] (高) | 1.690 | 5.050 | 1.690 |
| Rmerge_l_obs | 0.055 | 0.035 | 0.685 |
| Rmeas | 0.057 | 0.037 | 0.722 |
| Total number of observations | 242635 | ||
| 独立反射数 | 23647 | 892 | 3810 |
| <I/σ(I)> | 22.54 | 57.71 | 3.12 |
| 完全性 [%] | 99.8 | 100 | 98.7 |
| 冗長性 | 10.261 | 10.483 | 9.813 |
| CC(1/2) | 0.999 | 0.999 | 0.928 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | Potassium phosphate dibasic, HEPES pH7.5, Sodium phosphate monobasi |
