6KYU
Complex assembly, crystallization and preliminary X-ray crystallographic studies of duck MHC class I molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-04-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 62 |
| Unit cell lengths | 82.468, 82.468, 112.507 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.810 - 1.500 |
| Rwork | 0.166 |
| R-free | 0.18780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.739 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.850 | 1.300 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.086 | 0.086 |
| Number of reflections | 101015 | 101015 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 86.3 | |
| Redundancy | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2% v/v Tacsimate TM pH 7.0 0.1 M HEPES pH 7.5 20% w/v Polyethylene glycol 3,350 |
