6KYI

Rice Rubisco in complex with sulfate ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-30
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	110.061, 110.061, 199.748
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.030 - 1.750
R-factor	0.1369
Rwork	0.129
R-free	0.14330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3axm
RMSD bond length	0.003
RMSD bond angle	0.601
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.030	1.813
High resolution limit [Å]	1.750	1.750
Rmerge	0.104	0.887
Rmeas	0.107	0.927
Rpim	0.028	0.266
Number of reflections	118922	11805
<I/σ(I)>	23.31	2.85
Completeness [%]	99.9	99.26
Redundancy	14.9	11.7
CC(1/2)	0.999	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M Tris-HCl (pH 9.0), 0.2 M lithium sulfate, 15 % (w/v) polyethylene glycol 4000, and 10 % (w/v) glycerol