6KYF

Crystal structure of an anti-CRISPR protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 62 2 2
Unit cell lengths	104.677, 104.677, 119.673
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.950 - 3.070
R-factor	0.2056
Rwork	0.205
R-free	0.22380
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.716
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.070	3.070
Number of reflections	7640	716
<I/σ(I)>	29.7
Completeness [%]	99.5
Redundancy	13.8
CC(1/2)	0.976	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	HEPES, sodium citrate