6KY3

Structure of arginine kinase H284A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-26
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	77.943, 57.840, 74.860
Unit cell angles	90.00, 100.46, 90.00

Refinement procedure

Resolution	46.170 - 1.340
R-factor	0.17572
Rwork	0.173
R-free	0.21828
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bhl
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.170	1.410
High resolution limit [Å]	1.340	1.340
Rmerge	0.038	0.370
Number of reflections	70591	9870
<I/σ(I)>	16.1
Completeness [%]	96.6
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.8M phosphate buffer