6KXA
Galectin-3 CRD binds to GalA dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.876, 57.276, 61.867 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.403 - 1.230 |
R-factor | 0.1674 |
Rwork | 0.166 |
R-free | 0.18450 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.011 |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.403 | 1.250 |
High resolution limit [Å] | 1.230 | 1.230 |
Rmerge | 0.047 | 0.628 |
Number of reflections | 37730 | 1853 |
<I/σ(I)> | 18.1 | |
Completeness [%] | 99.8 | |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG |