6KWJ
A Crystal Structure of OspA mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-08 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 128.501, 32.734, 72.247 |
Unit cell angles | 90.00, 100.28, 90.00 |
Refinement procedure
Resolution | 19.900 - 1.940 |
R-factor | 0.2032 |
Rwork | 0.201 |
R-free | 0.24530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g8c |
RMSD bond length | 0.006 |
RMSD bond angle | 0.781 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 1.990 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.049 | 0.440 |
Number of reflections | 22185 | 1123 |
<I/σ(I)> | 28.2 | 11.6 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 3.2 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 282 | 26%PEG 400, 0.1M Imidozole, 16.1 mg/mL |