6KWJ
A Crystal Structure of OspA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-08 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.501, 32.734, 72.247 |
| Unit cell angles | 90.00, 100.28, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.940 |
| R-factor | 0.2032 |
| Rwork | 0.201 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8c |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.781 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.049 | 0.440 |
| Number of reflections | 22185 | 1123 |
| <I/σ(I)> | 28.2 | 11.6 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 282 | 26%PEG 400, 0.1M Imidozole, 16.1 mg/mL |
