6KWC
Crystal Structure Analysis of Endo-beta-1,4-xylanase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2019-06-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.495, 59.185, 69.841 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 1.300 |
| R-factor | 0.158 |
| Rwork | 0.157 |
| R-free | 0.17200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dfc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.014 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.920 | 1.340 |
| High resolution limit [Å] | 1.297 | 1.300 |
| Rmerge | 0.190 | 0.190 |
| Number of reflections | 49236 | |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 97.5 | |
| Redundancy | 2.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.5 | 291 | PEG 8000, NaI, NaAc-HAc |
