6KWB

AtDAO1(dioxygenase for auxin oxidation 1 from Arabidopsis thaliana) - 2-oxoglutarate binray complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-09-13
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 21 21 21
Unit cell lengths	75.880, 76.799, 166.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.208 - 2.518
R-factor	0.22810200341
Rwork	0.225
R-free	0.28153
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kwa
RMSD bond length	0.010
RMSD bond angle	1.489
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.570
High resolution limit [Å]	2.480	2.480
Rmerge	0.126	3.770
Number of reflections	34716	3206
<I/σ(I)>	11.6
Completeness [%]	98.8
Redundancy	7.1
CC(1/2)	0.996	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	0.03M KH2PO4, 16% PEG 8000, 20% Glycerol